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© Liliana Mancio, Institut Pasteur
Primary human hepatocytes co-cultured with parenchymal cells at 6 days post-seeding. The expression of human CD81 is depicted in pink
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Cell host & microbe - 13 Jul 2022

Mancio-Silva L, Gural N, Real E, Wadsworth MH, Butty VL, March S, Nerurkar N, Hughes TK, Roobsoong W, Fleming HE, Whittaker CA, Levine SS, Sattabongkot J, Shalek AK, Bhatia SN,

Link to Pubmed [PMID] – 35443155

Link to DOI – S1931-3128(22)00164-010.1016/j.chom.2022.03.034

Cell Host Microbe 2022 07; 30(7): 1048-1060.e5

Malaria-causing Plasmodium vivax parasites can linger in the human liver for weeks to years and reactivate to cause recurrent blood-stage infection. Although they are an important target for malaria eradication, little is known about the molecular features of replicative and non-replicative intracellular liver-stage parasites and their host cell dependence. Here, we leverage a bioengineered human microliver platform to culture patient-derived P. vivax parasites for transcriptional profiling. Coupling enrichment strategies with bulk and single-cell analyses, we capture both parasite and host transcripts in individual hepatocytes throughout the course of infection. We define host- and state-dependent transcriptional signatures and identify unappreciated populations of replicative and non-replicative parasites that share features with sexual transmissive forms. We find that infection suppresses the transcription of key hepatocyte function genes and elicits an anti-parasite innate immune response. Our work provides a foundation for understanding host-parasite interactions and reveals insights into the biology of P. vivax dormancy and transmission.

https://pubmed.ncbi.nlm.nih.gov/35443155