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© Robust Optical density measurement of cell count after calibration with serial Silica beads dilution. iGEM Pasteur_Paris 2018
Publication : Proceedings of the National Academy of Sciences of the United States of America

A packing for A-form DNA in an icosahedral virus

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Proceedings of the National Academy of Sciences of the United States of America - 21 Oct 2019

Wang F, Liu Y, Su Z, Osinski T, de Oliveira GAP, Conway JF, Schouten S, Krupovic M, Prangishvili D, Egelman EH

Link to Pubmed [PMID] – 31636205

Proc. Natl. Acad. Sci. U.S.A. 2019 Nov;116(45):22591-22597

Studies on viruses infecting archaea living in the most extreme environments continue to show a remarkable diversity of structures, suggesting that the sampling continues to be very sparse. We have used electron cryo-microscopy to study at 3.7-Å resolution the structure of the polyhedral virus 1 (SPV1), which was originally isolated from a hot, acidic spring in Beppu, Japan. The 2 capsid proteins with variant single jelly-roll folds form pentamers and hexamers which assemble into a = 43 icosahedral shell. In contrast to tailed icosahedral double-stranded DNA (dsDNA) viruses infecting bacteria and archaea, and herpesviruses infecting animals and humans, where naked DNA is packed under very high pressure due to the repulsion between adjacent layers of DNA, the circular dsDNA in SPV1 is fully covered with a viral protein forming a nucleoprotein filament with attractive interactions between layers. Most strikingly, we have been able to show that the DNA is in an A-form, as it is in the filamentous viruses infecting hyperthermophilic acidophiles. Previous studies have suggested that DNA is in the B-form in bacteriophages, and our study is a direct visualization of the structure of DNA in an icosahedral virus.

https://www.ncbi.nlm.nih.gov/pubmed/31636205