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© Research
Publication : Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII

A new method to achieve tens of nm axial super-localisation based on conical diffraction PSF shaping

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII - 25 Jan 2015

Clément Fallet, Maxime Dubois, Jean-Yves Tinevez, Stephane Oddos, Julien Caron, Roger Persson, Spencer L Shorte, Gabriel Y Sirat

SPIE BiOS, 2015

We present a method for Z-super-localization in fluorescence microscopy, based on conical diffraction. By using a thin biaxial crystal, the Point Spread Function (PSF) shape of an objective is made to depend strongly on the z coordinate. This z dependence is then exploited to localize fluorescent emitters axially with a great precision. We study how this method can be used for single molecule imaging with a global assessment by Fisher information analysis. Preliminary experiments demonstrate that this technique can obtain resolutions of tens of nm with the use of high NA objectives.

http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2197864