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© Research
Publication : Chemical communications (Cambridge, England)

A method for selecting modified DNAzymes without the use of modified DNA as a template in PCR

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Chemical communications (Cambridge, England) - 25 Jan 2015

Renders M, Miller E, Hollenstein M, Perrin D

Link to Pubmed [PMID] – 25487111

Chem. Commun. (Camb.) 2015 Jan;51(7):1360-2

Modified DNAzyme selections typically depend on recopying catalytically active modified DNA (mDNA) into cDNA in a PCR amplification step. However mDNA is often a poor template in PCR. Herein we propose a selection method in which the catalytically active, mDNA strand is covalently linked to the unmodified DNA template strand from which it was polymerized. Following selection, the unmodified DNA template is amplified in a PCR instead of the mDNA. This method circumvents the PCR amplification of mDNA.

http://www.ncbi.nlm.nih.gov/pubmed/25487111