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© Research
Publication : Carbohydrate research

1H- and 13C-n.m.r. assignments and conformational analysis of some monosaccharide and oligosaccharide substrate-analogues of lysozyme

Team: Archived: Nuclear Magnetic Resonance Of Biomolecules
Member: Muriel Delepierre
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Carbohydrate research - 15 Jun 1985

Boyd J, Porteous R, Soffe N, Delepierre M

Link to Pubmed [PMID] – 4028052

Carbohydr. Res. 1985 Jun;139:35-46

The 1H- and 13C-n.m.r. spectra of solutions of GlcNAc, beta-GlcNAc-(1—-4)-GlcNAc, and beta-GlcNAc-(1—-4)-beta-GlcNAc-(1—-4)-GlcNAc in D2O at 50 degrees are interpreted in terms of the conformations, using a combination of 1D- and 2D-n.m.r. spectroscopy and spectra simulation techniques. Two preferred orientations of the hydroxymethyl group were found for each of these saccharides. The conformations have been compared with those found from X-ray crystallographic data and conformational energy calculations.