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© Research
Project

Targeted Transgenesis By Homologous Recombination In Embryonic Stem (ES) Cells

Team: Mouse Genetics Engineering
Member: Francina Langa Vives
Member: Carine Moigneu
Member: Gaëlle Chauveau-Le Friec
Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
26
Jan 2015
Status
Ongoing
Members
3
Structures
1

About

A gene targeting vector is transfected into the desired mouse ES cells to modify an endogenous gene by homologous recombination. The mutant ES cells thus obtained are subsequently microinjected into mouse blastocysts for the generation of germ-line chimeras. Together with  “classical” 129-derived ES cell lines (i.e.129Sv, 129 Ola, AB1, TC1, CK35Rosa ERT2) we perform currently gene targeting projects using mainly C57BL/6-derived ES cells (C57BL/6N, C57BL/6J).