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  • Full Professor
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  • Physician
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  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
10
Feb 2015
Status
Ongoing
Members
1
Structures
1

About

Electron tomography (ET) is one of the two EM methods to obtain three- dimensional ultra-structural images. It relies on tilting the specimen and the automated acquisition of multiple tilt projections. It overcomes one of the major limitations of thin sections TEM that is restricted to 2D-images.

proj4
Study of Shigella flexneri infection of Hela cells by 3D Correlative Light-Electron Tomography: in order to identify and image the subsequent entry steps of Shigella into host cells, we developed an innovative methodology that combines correlative light and transmission electron microscopy with serial sectioning and tomography on identical samples. The final aim is to couple the functional information derived by 3D confocal microscopy with the high-resolution structural information from 3D electron tomography. Bacteria entry events identified by actin foci were imaged in 3D by confocal microscopy (fig.A, boxed area: bacteria are labelled in red and GFP-actin in green), processed for transmission electron microscopy and the same entry event were then imaged in 3D by electron tomography (fig.B). The reconstructed tomographic volume was then modelled in 3D (fig.C & D), highlighting novel vesicular structures that are docking or fusing with the phagocytic vacuole. (Scale bars: 1 µm)