Cilia are highly conserved organelles present in most eukaryotic cell types. The transition zone (TZ) is a ciliary subdomain that acts as a “gate” to control the composition of the cilium. The importance of the TZ is reflected in the many human diseases (termed ciliopathies) that are caused by mutations in TZ complexes. Here, we use a new proteomics technique to find new components of the African trypanosome TZ. We leverage the extraordinary tractability of this system to investigate TZ proteins, localizing them to distinct subdomains within the TZ, and demonstrating their essential roles in building cilia. We show that while orthologs of some ciliopathy complexes show long-term association with the TZ, others are highly dynamic.
Site web du laboratoire : http://www.sdeanresearch.com/
Contact : Philippe BASTIN (email@example.com – Poste : 38.35)