Date
22
Feb 2018
Time
14:00:00
Institut Pasteur, Paris, France
Address
Building: Lwoff
Location
2018-02-22 14:00:00
2018-02-22 15:00:00
Europe/Paris
C3BI Seminar – Inferring causality in complex molecular pathways from live cell movies
One of the major limitations in the study of complex molecular pathways is adaptation of the system to experimental perturbation. With ‘complex’ we mean pathways with a significant level of functional redundancy between components […]
Institut Pasteur, Paris, France
Olaya Rendueles-Garcia
olaya.rendueles-garcia@pasteur.fr
About
One of the major limitations in the study of complex molecular pathways is adaptation of the system to experimental perturbation. With ‘complex’ we mean pathways with a significant level of functional redundancy between components and nonlinear interactions. In this scenario, perturbation of one component may lead to an observable phenotype. However, it is impossible to interpret the difference between phenotype and wildtype in terms of the function the targeted component fulfills in the unperturbed system – although this is the predominant approach cell and systems biologists take to dissect molecular functions. Over the past decade my lab has made efforts to circumvent this problem by exploiting the basal fluctuations of molecular activities observed in live cell movies to establish causal functional relations between pathway components. Inspired by the accomplishments of econometrics, where predictive models are built entirely from passive observation of financial fluctuation time series, we have developed a computational framework to determine nonlinear interdependencies between pathway components. This presentation will introduce some of the mathematical, computational, and experimental concepts based on which we can now accurately delineate the functional hierarchy between signaling and mechanical processes that control cellular morphogenesis, which is a prime example of a complex molecular process
Location
Building: Lwoff
Address: Institut Pasteur, Paris, France