Inverted widefield Microscope equipped with LED colibris and mercury lamp allowing acquisition on live fluorescent samples with control of the focus using the ‘Definite Focus’ module.
Features :
Available light sources
- Mercury Lamp HXP120
- LED colibri
- 365 nm
- 470 nm
- 555 nm
- 590 nm
Available filters
| Carousel # | Example fluorophores | Excitation | Dichroic | Emission | Commercial name |
| #1 | DAPI, Hoechst, Alexa Fluor 405, DyLight 405 | BP 359-371 | FT 395 | BP 421-479 | Zeiss FS01, modified emission |
| #2 | eGFP | BP 450-490 | FT 495 | BP 500-550 | Zeiss FS38 HE |
| #3 | Cy3, TRITC, Alexa Fluor 546, Alexa Fluor 555, DsRed | BP 540-552 | FT 550 | BP 565-595 | Omega XF37 |
| #4 | Cy5, Alex Fluor 633, Alex Fluor 647, DRAQ5 | BP 605-645 | FT 650 | BP 668-723 | Omega XF110-2 |
| #5 | Interferential Contrast | DIC | |||
| #6 | HcRed, TexasRed | BP 576-596 | FT 605 | BP 668-723 | Semrock HcRed |
Objectives
- 10X : PlnN 10X/0.3 Ph 1
- 20X : LD Achroplan 20X/0.4 Ph 2
- 25X : PlnN 25X/0.8 Oil-W-GlycPh 2
- 40X : Pln-Apo 40X/1.3 oil Ph3
- 63X : Pln-Apo 63X/1.4 oil Ph3
- 100X : PlnN 100X/1.4 oil
Detectors
- Dual camera sCMOS Hamamatsu ORCA FLASH 4.0 v2.0
- Zeiss Definite Focus as hardware focus stabilization.
- motorized XY stage MAC 5000
- Stand: Zeiss Axio Observer Z1 with touch screen
- Environmental chamber with temperature, CO2 and O2 control
- Acquisition software : ZEN blue 2012
Capabilities:
- Multi Channel
- Measurement
- Z-Stack
- Time series
- Tiles
- Panorama
- Experiment Designer
- Software Autofocus