This particular system is equipped with two of the major super resolution modalities Lattice SIM (Structured Illumination Microscopy) and 3D SMLM (Single Molecule Localisation Microscopy) allowing the observation of fixed and living biological phenomena at spatial and temporal resolution scales not accessible with conventional microscopes. ( Achievement of a lateral precision of localisation of 10-20nm using SMLM, and using the other modality Lattice SIM a temporal resolution up to 19 images/sec). The microscope is also equipped with 2 cameras, an incubator, a CO2 controller and a drift correction system offering ideal conditions for live imaging.
Features :
Available laser lines for excitation
- 405 nm – 50 mW
- 488 nm – 500 mW
- 561 nm – 500mW
- 642 nm – 500 mW
- Fluorescent Lamp HXP 120
Objectives
- 10X/0.3 : EC Plan Neofluar
- 20X/0.8 : Plan Apochromat
- 40X/1.4 : Plan Apochromat
- 63X/1.4 : Plan Apochromat
- 63X/1.46 : Plan Apochromat
Filters for observation and acquisition :
- Observation : Multiband Filter 25 HE
- Acquisition :
- Quad band filter
- BP 420-480
- BP 495-590
- LP 570
Detectors-
- 2 cameras PCO Edge 4.2 QE 82%
- XY Piezo scanning stage
- z-piezo stage
- Focus control by Definite focus 2
- temperature and CO2 control
- Stand: Zeiss Axio observer 7 with touch screen
- Acquisition software : ZEN
Capabilities:
- Lattice SIM
- Apotome
- dual Camera
- TIRF
- SMLM 3D
The Super Resolution microscope, “Elyra 7” from Carl Zeiss Gmbh (Jena, Germany) have been partially financed by Institut Pasteur, Labex IBEID and DIM1HEALTH