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© Pierre Lafaye
Astrocytes marqués par des anticorps VHH anti-GFAP. Des anticorps d'alpagas dirigés contre une protéine spécifique des astrocytes, la GFAP (Glial Fibrillary Acidic Protein), ont été obtenus à partir de camélidés immunisés. La partie VHH (partie de l'anticorps qui reconnaît l'antigène) a été exprimée sous forme recombinante chez Escherichia coli.
Publication : Transactions of the Royal Society of Tropical Medicine and Hygiene

Laboratory evaluation of a rapid diagnostic test for Neisseria meningitidis serogroup A.

Team: Antibody Engineering
Team: Invasive Bacterial Infections
Team: Enteric Bacterial Pathogens
Member: Pierre Lafaye
Member: Muhamed-Kheir Taha
Member: Jean-Marc Collard
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Transactions of the Royal Society of Tropical Medicine and Hygiene - 01 Jul 2013

Terrade A, Collard JM, Nato F, Taha MK

Link to Pubmed [PMID] – 23687106

Link to DOI – 10.1093/trstmh/trt041

Trans R Soc Trop Med Hyg 2013 Jul; 107(7): 460-1

Epidemics of meningococcal meningitis occur periodically in the African ‘meningitis belt’ and are mainly, but not only, due to serogroup A.We tested a dipstick as a rapid detection test (RDT) to detect serogroup A using 401 cerebrospinal fluid (CSF) samples.The detection limits were 10(5) CFU/ml with sensitivity and specificity for detecting serogroup A in CSF samples of 88% and 99%, respectively.The new RDT can be used in field surveillance of meningococcal meningitis to help characterize meningitis cases particularly after introduction of the conjugate vaccine against serogroup A.