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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : The Journal of infectious diseases

Flowcytometric and ImageStream Rna-Fish Gene Expression, Quantification and Phenotypic Characterization of Blood Sporozoites and Sporozoites From Human Malaria Species.

Team: Biology of Host-parasite Interactions
Team: Group: Humanized mice and in vivo analysis of human Plasmodium infections
Member: Sabine Thiberge
Member: Malika Serra-Hassoun
Member: Artur Scherf
Member: Sylvie Garcia
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of infectious diseases - 28 Aug 2021

Luiza-Batista C, Nardella F, Thiberge S, Serra-Hassoun M, Ferreira MU, Scherf A, Garcia S,

Link to Pubmed [PMID] – 34453537

Link to DOI – 10.1093/infdis/jiab431

J Infect Dis 2022 05; 225(9): 1621-1625

We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.