Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search

← Go to Research

Go back
Scroll to top
Share
Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
08
Jan 2021
Status
Completed
Members
6
Structures
2
Publications
5

About

Entamoeba histolytica is a protozoan parasite and an amitochondriate pathogenic amoeba, which may cause dysentery and liver abscess in humans (i.e. amoebiasis). Differential pathogenicity is observed among E. histolytica strains: strain HM1:IMSS is virulent while strain Rahman is naturally attenuated. A closely related species, Entamoeba dispar, is non-pathogenic and produces asymptomatic infections. Another species, Entamoeba moshkovskii, lives mostly freely and rarely infects humans. Despite the fact that these Entamoeba species are morphologically indistinguishable and phylogenetically closely related, their clinical results are radically different. Their phenotypic differences provide an excellent theoretical basis for comparative analyzes at the genome level to search for factors relevant to pathogenicity and adaptation to humans.
Our aim is to understand the phenotypic differences between Entamoeba species/strains using comparative phylogenomic and transcriptomic approaches, which is supported by two primary objectives; 1) To catalogue the relevant and high-resolution transcriptomic and phylogenomic differences between Entamoeba species/strains and 2) To functionally annotate the Entamoeba protein families and discover the relevant gene sets relevant for the phenotypic differences between Entamoeba species/strains.
Taking advantage of the next-generation sequencing technologies, we characterize the transcriptional landscape of the Entamoeba species/strains at an unprecedented scale and resolution, including the generation of genome wide maps for coding and non-coding transcripts, small RNAs and anti-sense RNAs, as well as the expression profiles of these transcripts in three culture conditions (axenic culture, nitric oxide treatment and ex vivo colon culture). By discovering the diversity and expression profiles of small RNAs and anti-sense RNAs we expect to provide valuable insights into potential roles of non-coding RNA across the Entamoeba transcriptomes.
Genamibe represents the first and the most comprehensive study on Entamoebidae in terms of : 1) the types of transcripts we are able to capture; 2) the quality of the coding transcript map ( i.e. map of transcription start site, splicing junctions, alternative splicing pattern and poly-adenylation sites); 3) the scope of comparisons (i.e. inter-species, inter-strain and inter-culture-condition comparisons).
This study has a significant impact on the fundamental understanding of transcription in lower eukaryotes and set an example for high-quality standard for studies in similar kinds of protists.

Fundings

References