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© Research
Publication : PLoS neglected tropical diseases

Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro

Team: Trypanosomatids Infectious Processes
Member: Thierry Lang
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in PLoS neglected tropical diseases - 13 Sep 2011

Michel G, Ferrua B, Lang T, Maddugoda MP, Munro P, Pomares C, Lemichez E, Marty P

Link to Pubmed [PMID] – 21931877

PLoS Negl Trop Dis 2011 Sep;5(9):e1323

Here we engineered transgenic Leishmania infantum that express luciferase, the objectives being to more easily monitor in real time their establishment either in BALB/c mice–the liver and spleen being mainly studied-or in vitro. Whatever stationary phase L. infantum promastigotes population–wild type or engineered to express luciferase-the parasite burden was similar in the liver and the spleen at day 30 post the intravenous inoculation of BALB/c mice. Imaging of L. infantum hosting BALB/C mice provided sensitivity in the range of 20,000 to 40,000 amastigotes/mg tissue, two tissues-liver and spleen-being monitored. Once sampled and processed ex vivo for their luciferin-dependent bioluminescence the threshold sensitivity was shown to range from 1,000 to 6,000 amastigotes/mg tissue. This model further proved to be valuable for in vivo measurement of the efficiency of drugs such as miltefosine and may, therefore, additionally be used to evaluate vaccine-induced protection.