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  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
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© Research
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in - 07 Nov 2023

Karine Nozeret, Nienke Buddelmeijer

Link to HAL – hal-04370821

Link to DOI – 10.1007/978-1-0716-3445-5_5

E. Cascales; L. Journet. Bacterial Secretion Systems, 2715, Springer US, pp.79-89, 2023, Methods in Molecular Biology, 978-1-0716-3444-8. ⟨10.1007/978-1-0716-3445-5_5⟩

Lipoproteins contain fatty acids at their amino termini through which they are anchored in lipid membranes. Here, we describe the use of alkyne fatty acids and click chemistry to label lipoproteins in bacterial cells. Exogenous fatty acids containing an alkyne group are incorporated in phospholipids and lipoproteins during bacterial growth. Protein precipitation followed by click chemistry renders lipoproteins fluorescent that can be visualized by in-gel fluorography. Alkyne phospholipids that are the acyl donor in the maturation process of lipoproteins can also be isolated from bacterial membranes for further enzymatic and kinetic studies of acyltransferase that use phospholipids as substrate.