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Présentation

News

👩‍🔬We are pleased to announce that the training: Introduction to the basics of Histology and Immunostaining will take place from October 7 to 11, 2024 👨‍🔬
You can Register now 😁

Introduction to the basics of Histology and Immunostaining


About us

Our platform offers state-of the art large histotechnological laboratory with a specialized team. We are available for collaborative research. Many projects are currently ongoing on animal models of infectious diseases, cancer, immunotherapy or inflammation with internal or external research teams. Individuals wishing to develop collaborative research efforts are encouraged to contact us when developing research projects. Optimal results are obtained when the staff is contacted as early as possible in the conception of the in vivo part of the project; this allows us to help determine how histological approach can best support the research aim.

To find out more see the Technology Department brochure


Requests

For all requests, please download and complete the samples form

To request a training or reserve a system: PPMS


Range of services

A variety of histology services can be provided to research investigators:

  • Necropsy & gross examination of animal tissues
  • Design of the protocol
  • Methodology advice on proper tissue collection
  • Choice of morphological approaches (fixation, embedding, slide preparation, slide treatments)
  • Evaluation of the slides
  • Histological and morphometric analyze
  • Fine tuning of Histopathology protocols
  • Discussion of the results
  • Recommendations for further experiments

Technics

Our Histology laboratory prepares slides and a wide variety of quality, flexible histopathological and immunohistochemical stains for investigative staff. We also develop new technical tools for animal tissue analysis:We also have access to Safety level 3 facility for frozen sectioning of infected human or animal tissues.


Access protocol


Sample process :


Equipment :


Terms of collaboration/authorship

Engineers and Pathologists who contributed to the project must be listed as co-authors and Technicians in acknowledgement.

Équipements

Booking site

Sliding Microtome (refrigerated head) • Leica • SM2010 R

With smooth movement of the sledge, precision specimen orientation, and emphasis on safety and ergonomics, the SM2010 R produces high quality sections for routine histopathology (paraffin).The stable sliding microtome has a totally enclosed micrometer […]

Slide scanner • Zeiss • AxioScan 7

The Axio Scan 7 allow to digitize histological sections and  create high-quality virtual slides. Both bright field and fluorescent sections can be digitalized. Virtual slides can be then be kept and observed or used […]

2 • Cryostat • Leica • CM1950

This material is needed  to perform frozen sections.  People trained in frozen sectioning  from other laboratories can use this material under our supervision. Please contact us 

2 • Bond RX Leica • Leica • Bond RX

BOND RX fully automated research stainer Make that next big breakthough with confidence. From IHC to ISH, FISH, CTC, Multiplexing and more, the BOND RX provides research labs with an easy way to quickly […]

Automated tissue processor • LEICA • TP 1050

This material is needed for routinely dehydration and pre-embedding of tissue samples in paraffin. We can perform the pre-embedding of your tissue samples Please contact us if needed

Digital camera • NIKON • DSRi2

High quality bright field microphotographs can taken with this material, which is connected to a NIKON 50i microscope. People from other laboratories can have access to this equipment Please contact us if needed  

Vibratome • LEICA • VT 1200S

Vibrating microtomes (vibratomes) allows to cut tissues without freezing or embedding. 50-100 um sections can be performed to study the tissue morphology in 3D or to preserve enzyme and cell activities within thick slices. […]

2 • Cryostat • LEICA • CM 3050S

This material is needed  to perform frozen sections.  People trained in frozen sectioning  from other laboratories can use this material under our supervision. Please contact us 

Automated slide stainer • Leica • ST5020

This automat provides reproducible automated, high quality standard stainings for paraffin or frozen histological sections. Please contact us if you need to stain histological sections (Hematoxylin-Eosin or special stains) Please contact us if needed

4 • Microtome • LEICA • RM 2245

A microtome is needed to perform paraffin sections. Trained people from other laboratories can have access to this equipment under our supervision Please contact us if needed  

2 • Paraffin embedding station • LEICA • EG 1150

The paraffin embedding station is needed to routinely prepare paraffin blocks. We can perform the embedding of your tissue samples Please contact us if needed

Slide scanner • ZEISS • Axio Scan.Z1

The Axio Scan allow to digitize histological sections and  create high-quality virtual slides. Both bright field and fluorescent sections can be digitalized. Virtual slides can be then be kept and observed or used for […]

Anciens Membres

2000
2000
Name
Position
2019
2023
Johan Bedel
Technician

Cours

Introduction to the basics of Histology and Immunostaining

Program

2024-10-07 09:00:00 2024-10-11 16:00:00 Europe/Paris Introduction to the basics of Histology and Immunostaining Program Institut Pasteur, Rue du Docteur Roux, Paris, France

Introduction to the basics of Histology and Immunostaining

Objectives : – Acquire basics in histology in order to know the sample preparation process – Know the different principles of histology – Understand immunochemistry & immunofluorescence techniques Program : – Sample preparation – […]

2023-10-09 09:00:00 2023-10-13 16:00:00 Europe/Paris Introduction to the basics of Histology and Immunostaining Objectives : – Acquire basics in histology in order to know the sample preparation process – Know the different principles of histology – Understand immunochemistry & immunofluorescence techniques Program : – Sample preparation – […] 28 Rue du Docteur Roux, Paris, France

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