Lien vers Pubmed [PMID] – 8673615
C. R. Acad. Sci. III, Sci. Vie 1996 Jan;319(1):1-7
The 8-hydroxyguanine:adenine mispairing scheme that spontaneously occurs in vivo through oxidative metabolism of DNA was edited to obtain a pair closely fitting the Watson-Crick geometry in which 2 purine bases of identical structure but oppositely rotated in the syn and anti configurations are hydrogen-bonded. The structure thus designed, 2-amino-8-hydroxypurine, was synthesized as a DNA precursor from deoxyguanosine in 8 steps by oxidation at carbon 8 of guanine followed by reduction at carbon 6. Polydeoxynucleotides embodying this self-complementary base are expected to undergo direct copying processes through polymerase catalysis.