Lien vers Pubmed [PMID] – 2671680
Mol. Cell. Probes 1989 Jun;3(2):159-66
Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybridization with non-radioactive probes and hybridization with radiolabelled probes were 2 ng, 0.2 ng, 0.02 ng of purified virus, respectively. Dot hybridization with non-radioactive probes could be performed on crude infected beet root extracts, thus providing a useful tool for monitoring BNYVV infection and for routine testing in plant breeding programs.