Cryo-EM of vitreous sections (CEMOVIS) is the method of choice to study native structures. Samples are preserved by rapid freezing, cryo-sectioned and observed by cryo-TEM without further contrasting and embedding.
CEMOVIS section of Sulfolobus islandis infected by Sulfolobus islandicus rod shaped virus 2 (SIRV2) imaged by cryo-EM at low dose conditions using a FEI Tecnai with field emission gun operated at 200kV. Virions form clusters in the cytoplasm of the infected cell here shown as cross-section. The insert is a higher magnification showing structural details of the cluster. Scale bar: 200 nm. Collaboration with David Prangishvilli, Biologie Moléculaire du Gène chez les Extrêmophiles
