Présentation
Joe Bondy Denomy
The University of California, San Fransisco
«How bacteriophages protect themselves from CRISPR»
Monday November 26th 2018 at 11:00
Auditorium Jacques Monod
Bacterial CRISPR-Cas systems utilize sequence-specific RNA-guided nucleases to defend against bacteriophage (phage) infection. As a counter-measure, numerous phages produce proteins to block the function of the Class 1 CRISPR-Cas systems, which utilize multi-subunit protein complexes to enact immunity. Our group recently developed and utilized novel bioinformatics strategies to identify Class 2 (e.g. Cas9 and Cas12) anti-CRISPR proteins. We have identified anti-CRISPR proteins encoded by phages and mobile genetic elements from many organisms, including Pseudomonas aeruginosa, Listeria monocytogenes, Moraxella bovoculi, and Streptococcus pyogenes, suggesting widespread and common CRISPR-Cas inactivation. More recently, we have identified a phage with a novel mechanism of CRISPR evasion that does not rely on anti- CRISPR proteins, but instead physically segregates its genome from nucleases. I will discuss our progress towards understanding these variousmechanisms that phages deploy to avoid destruction by CRISPR-Cas. This work is multidisciplinary and unites fields spanning microbial/phage genetics, molecular biology, phage biology, structure, bioinformatics, gene editing, and cell biology
contact :
Ivo GOMPERTS BONECA (ivo.gomperts-boneca@pasteur.fr)
Localisation
Bâtiment: Monod
Adresse: 25-28 Rue du Docteur Roux, Paris, France
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