Lien vers Pubmed [PMID] – 9461480
Lien vers HAL – univ-guyane-03289316
Lien DOI – 10.1093/nar/26.4.1130
Nucleic Acids Research, 1998, 26 (4), pp.1130-1131. ⟨10.1093/nar/26.4.1130⟩
A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.