High-Throughput Crystallization Screening

Présentation

Application process of crystallization at 18°C or 4°C

In the platform we can setup high-throughput crystallization experiments (18°C or 4°C) with the vapour diffusion (sitting or hanging drop), the microbatch, and the cubic phase methods. The screening can be made with the Mosquito or with the Douglas ORYX-8 robots. These robots can dispense drops from 100nl to 2µl. The crystallization plates will then be stored at 18°C or 4°C in a Formulatrix® Robots (an automated imaging system for protein crystallization). The crystallization drops will automatically be imaged every day. If successful results are obtained, the crystals will be reproduced and optimized using crystallization solutions generated by a Matrix Maker automated formulation system (Emerald BioSystems®) (see the Matrixmaker service).

Bring the proteins to the Platform with the filled form

Kits available on the Platform

The initial screening is 6 or 7 plates of 96 different conditions among the following kits:

Kit Crystal screen 1 and 2 – Hampton: HR2-110 and HR2-112

Kit Wizard 1 and 2 – Jena Biosciences: CS-311 and CS-312

Kit MDL 1 and 2 – Molecular dimensions:

Kit Structure screen 1 – MD1-01

Kit Structure screen 2 – MD1-02

Kit JBS1-4 –Jena Biosciences:

JBS Screen 1 bulk    – CS-101L

JBS Screen 2 bulk    – CS-102L

JBS Screen 3 bulk    – CS-103L

JBS Screen 4 bulk    – CS-104L

Kit JBS 5-8 – Jena Biosciences:

JBS Screen 5 bulk – CS-105L

JBS Screen 6 bulk – CS-106L

JBS Screen 7 bulk – CS-107L

JBS Screen 8 bulk – CS-108L

Kit Salt RX HT – Hampton: HR2-108

Kit PEGion 1 and 2 – Hampton:

PEGion 1 Screen – HR2-126

PEGion 2 Screen – HR2-098

Crystal Screen Cryo 1 and 2:   HR2-122 and HR2-121

Kit Index – Hampton: HR2-144

Kit Proplex – Molecular dimensions: MD1-38

Nextal Classic suite – 1054292

Some other more specific kits at your disposal :

Kit Memstart/Memsys – Molecular dimensions: MD1-04ROWORLD:

Memstart – MD1-21

Memsys – MD1-25

Kit JBScreen Membrane 1,2,3, 4 – Jena Biosciences:

JBScreen membrane 1 – CS-301L

JBScreen membrane 2 – CS-302L

JBScreen membrane 3 – CS-303L

JBScreen membrane 4 – CS-304L

Kit MembFac – Hampton: HR2-114

Kit Natrix – Hampton: HR2-116

Kit memgold 1 – Molecular dimensions: MD1-39

Kit memgold 2 – Molecular dimensions: MD1-63

Additives Hampton – Hampton: HR2-428

Additives MEMadvantage: MD1-70

And some homemade kits:

PF6 DNA-PROTEIN

PF6 Additives Buffer-Salt_1

PF6 Additives

PF6 Additives Cryos 1

Un petit guide pour l'utilisation de la recherche avancée :

Présentation

Application process of crystallization at 18°C or 4°C

Protocole d'accès