High Content Analysis

System Characteristics

Available Objectives

• 5x air (NA 0.16, WD 12.1mm, ~ 2.583 μm x 2.583 μm).
• 10x air (NA 0.3, WD 5.2mm ~ 1.292 μm x 1.292 μm).
• 20x air (NA 0.4, WD 8.28mm ~ 646 um x 646 um)
• 40x air (NA 0.6, WD 2,9 mm ~ 323 μm x 323 μm).
• 40x water immersion (NA 1.1, WD 0,62 mm ~ 323 μm x 323 μm).
• 63x water immersion (NA 1.15, WD 0,6 mm ~ 205 μm x 205 μm).

Excitation lines

Unit equipped with the following solid state laser lines:
– 375 nm, 16 mW
– 425 nm, 100 mW
– 488 nm, 60 mW
– 561 nm, 50 mW
– 640 nm, 40 mW

Cameras

Two 16bits sCMOS cameras (4,7 megapixels (2160 x 2160)) with a 6,5 µm pixel size

Optical modes

Confocal and non confocal

Emission Filters

– 435-550 nm,

– 435-480 nm

– 500-550 nm

– 570-630 nm

– 650-760 nm

Incubation Chamber

Temperature control: 37^oC to 42^oC

CO₂ control: 1% to 10%