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© Research
Publication : The EMBO journal

Yeast DNA polymerase–DNA primase complex; cloning of PRI 1, a single essential gene related to DNA primase activity

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The EMBO journal - 01 Mar 1987

Lucchini G, Francesconi S, Foiani M, Badaracco G, Plevani P

Link to Pubmed [PMID] – 3034609

EMBO J. 1987 Mar;6(3):737-42

The immunopurified yeast DNA polymerase–DNA primase complex is constituted by DNA polymerase I polypeptides and by three other protein species, called p74, p58 and p48, which we show to be immunologically unrelated. The gene encoding the p48 polypeptide has been identified by immunological screening of a lambda gt11 yeast genomic DNA library. Antiserum specific for p48 inhibits DNA primase, and immunoreactive, inhibitory antibodies are affinity-purified by the clone-encoded protein, thus relating the p48 polypeptide to DNA primase activity. The entire gene has been cloned, and the 1.45-kb p48 mRNA is overproduced in cells containing the gene in high copy number. Gene disruption and Southern hybridization experiments demonstrate that the p48 protein is encoded by a single gene and it performs an essential function.

http://www.ncbi.nlm.nih.gov/pubmed/3034609