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© Research
Publication : Archives of biochemistry and biophysics

Validation methods for low-resolution fitting of atomic structures to electron microscopy data.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Archives of biochemistry and biophysics - 01 Sep 2015

Xu XP, Volkmann N,

Link to Pubmed [PMID] – 26116787

Link to DOI – 10.1016/j.abb.2015.06.017S0003-9861(15)30003-5

Arch Biochem Biophys 2015 Sep; 581(): 49-53

Fitting of atomic-resolution structures into reconstructions from electron cryo-microscopy is routinely used to understand the structure and function of macromolecular machines. Despite the fact that a plethora of fitting methods has been developed over recent years, standard protocols for quality assessment and validation of these fits have not been established. Here, we present the general concepts underlying current validation ideas as they relate to fitting of atomic-resolution models into electron cryo-microscopy reconstructions, with an emphasis on reconstructions with resolutions below the sub-nanometer range.

https://pubmed.ncbi.nlm.nih.gov/26116787