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© Research
Publication : Frontiers in immunology

Vaccine Containing the Three Allelic Variants of the Circumsporozoite Antigen Induces Protection in Mice after Challenge with a Transgenic Rodent Malaria Parasite

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Frontiers in immunology - 11 Oct 2017

Gimenez AM, Lima LC, Françoso KS, Denapoli PMA, Panatieri R, Bargieri DY, Thiberge JM, Andolina C, Nosten F, Renia L, Nussenzweig RS, Nussenzweig V, Amino R, Rodrigues MM, Soares IS

Link to Pubmed [PMID] – 29075260

Front Immunol 2017;8:1275

is the most common species that cause malaria outside of the African continent. The development of an efficacious vaccine would contribute greatly to control malaria. Recently, using bacterial and adenoviral recombinant proteins based on the circumsporozoite protein (CSP), we demonstrated the possibility of eliciting strong antibody-mediated immune responses to each of the three allelic forms of CSP (PvCSP). In the present study, recombinant proteins representing the PvCSP alleles (VK210, VK247, and -like), as well as a hybrid polypeptide, named PvCSP-All epitopes, were generated. This hybrid containing the conserved C-terminal of the PvCSP and the three variant repeat domains in tandem were successfully produced in the yeast . After purification and biochemical characterization, they were used for the experimental immunization of C57BL/6 mice in a vaccine formulation containing the adjuvant Poly(I:C). Immunization with a recombinant protein expressing all three different allelic forms in fusion elicited high IgG antibody titers reacting with all three different allelic variants of PvCSP. The antibodies targeted both the C-terminal and repeat domains of PvCSP and recognized the native protein on the surface of sporozoites. More importantly, mice that received the vaccine formulation were protected after challenge with chimeric sporozoites expressing CSP repeats of sporozoites (Pb/PvVK210). Our results suggest that it is possible to elicit protective immunity against one of the most common PvCSP alleles using soluble recombinant proteins expressed by . These recombinant proteins are promising candidates for clinical trials aiming to develop a multiallele vaccine against malaria.

https://www.ncbi.nlm.nih.gov/pubmed/29075260