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© Institut Pasteur
Cryptococcus neoformans
Publication : Journal of clinical microbiology

Use of microsatellite markers and gene dosage to quantify gene copy numbers in Candida albicans

Team: RNA Biology of Fungal Pathogens
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of clinical microbiology - 01 Mar 2005

Costa JM, Eloy O, Botterel F, Janbon G, Bretagne S

Link to Pubmed [PMID] – 15750114

J. Clin. Microbiol. 2005 Mar;43(3):1387-9

With microsatellite marker typing, the number of alleles must be known for calculation of allelic frequencies in the diploid Candida albicans for a given locus. We describe a gene dosage with a double real-time PCR. Such a dosage should also be useful in exploring the loss of heterozygosity in C. albicans.