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© Research
Publication : Applied optics

Ultrahigh-resolution full-field optical coherence tomography

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Applied optics - 10 May 2004

Dubois A, Grieve K, Moneron G, Lecaque R, Vabre L, Boccara C

Link to Pubmed [PMID] – 15143811

Appl Opt 2004 May;43(14):2874-83

We have developed a white-light interference microscope for ultrahigh-resolution full-field optical coherence tomography of biological media. The experimental setup is based on a Linnik-type interferometer illuminated by a tungsten halogen lamp. En face tomographic images are calculated by a combination of interferometric images recorded by a high-speed CCD camera. Spatial resolution of 1.8 microm x 0.9 microm (transverse x axial) is achieved owing to the extremely short coherence length of the source, the compensation of dispersion mismatch in the interferometer arms, and the use of relatively high-numerical-aperture microscope objectives. A shot-noise-limited detection sensitivity of 90 dB is obtained in an acquisition time per image of 4 s. Subcellular-level images of plant, animal, and human tissues are presented.

http://www.ncbi.nlm.nih.gov/pubmed/15143811