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© Institut Pasteur/Antoinette Ryter
Salmonella spp. Bactéries à Gram négatif, aérobies ou anaérobies facultatifs à transmission orofécale. Les salmonelles majeures (sérotype typhi et sérotype paratyphi) sont responsables des fièvres typhoïde et paratyphoïde chez l'homme uniquement ; les salmonelles mineures (sérotype typhimurium et sérotype enteritidis) sont impliquées dans 30 à 60 % des gastroentérites et toxiinfections d'origine alimentaire. Image colorisée.
Publication : Diagnostic microbiology and infectious disease

Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA.

Team: Enteric Bacterial Pathogens
Member: Jean-Marc Collard
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Diagnostic microbiology and infectious disease - 01 Mar 2019

Ouattara M, Whaley MJ, Jenkins LT, Schwartz SB, Traoré RO, Diarra S, Collard JM, Sacchi CT, Wang X

Link to Pubmed [PMID] – 30413354

Link to DOI – 10.1016/j.diagmicrobio.2018.10.008

Diagn Microbiol Infect Dis 2019 Mar; 93(3): 188-190

This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.