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© Research
Publication : Methods in Molecular Biology

Translation Analysis at the Genome Scale by Ribosome Profiling

Member: Rachel Legendre
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in Molecular Biology - 02 Jan 2016

Agnès Baudin-Baillieu, Isabelle Hatin, Rachel Legendre and Olivier Namy

Link to Pubmed [PMID] – 26483019

Methods Mol Biol. 2016;1361:105-24

Ribosome profiling is an emerging approach using deep sequencing of the mRNA part protected by the ribosome to study protein synthesis at the genome scale. This approach provides new insights into gene regulation at the translational level. In this review we describe the protocol to prepare polysomes and extract ribosome protected fragments before to deep sequence them.