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© Research
Publication : Developments in biological standardization

The use of monoclonal anti-idiotypic antibodies for studying antigen-specific T and B cell receptors

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Developments in biological standardization - 01 Jan 1984

Sommé G, Roth C, Bismuth G, Gougeon ML, Thèze J

Link to Pubmed [PMID] – 6335440

Dev. Biol. Stand. 1984;57:385-91

Eight syngeneic HP-id specific for anti-GAT antibodies are tested for their capacity to detect idiotypes on GAT-specific T cell lines. Their affinity constants are between 5 X 10(-7) and 10(-8) molar-1 which permit significant binding analysis. They detect idiotopes expressed in anti-GAT sera from all the strains of mice tested and by 14 BALB/c HP-GAT. In addition, the distribution of the corresponding idiotopes among all allogeneic HP-GAT allow the identification of two groups of idiotopes. Some idiotopes are expressed by a minority of HP-GAT; the other idiotopes are expressed by all of them. Nevertheless, in spite of the broad molecular and genetic distribution of these idiotopes on anti-GAT antibodies, they are not expressed by three GAT-specific T cell lines from different genetic origins.

http://www.ncbi.nlm.nih.gov/pubmed/6335440