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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Molecular and biochemical parasitology

The S-antigen of Plasmodium falciparum Palo Alto represents a new S-antigen serotype

Team: Biology of Host-parasite Interactions
Member: Denise Mattei
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular and biochemical parasitology - 15 Jan 1988

Mattei D, Langsley G, Braun-Breton C, Guillotte M, Dubremetz JF, Mercereau-Puijalon O

Link to Pubmed [PMID] – 3278225

Mol. Biochem. Parasitol. 1988 Jan;27(2-3):171-80

The S-antigen from the Palo Alto isolate of Plasmodium falciparum has been characterized. The partial sequence for the gene coding for this antigen (clone 281) reveals the presence of tandem repeats of eight amino acids which defines a new S-antigen serotype. Antibodies raised against the 281 recombinant clone reacted with a 140 kDa antigen by immunoblotting with parasite extracts and culture supernatants. The 140 kDa peptide was also identified by immunoprecipitation of metabolic labelled parasites. The 281 mouse antiserum was used to localize the antigen on parasite smears by indirect immunofluorescence assay and more precisely by immunoelectron microscopy. The S-antigen is localized within the parasitophorous vacuole. Furthermore, different isolates were examined for the presence of the Palo Alto S-antigen specificity.