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© Research
Publication : Journal of physiology and pharmacology : an official journal of the Polish Physiological Society

The major protein fraction of mouse milk revisited using proven proteomic tools

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of physiology and pharmacology : an official journal of the Polish Physiological Society - 01 Oct 2009

Boumahrou N, Andrei S, Miranda G, Henry C, Panthier JJ, Martin P, Bellier S

Link to Pubmed [PMID] – 19996491

J. Physiol. Pharmacol. 2009 Oct;60 Suppl 3:113-8

The PRM/Alf inbred mice exhibit a huge intestinal lengthening. Since milk contains bioactive factors implied in numerous biological processes, one hypothesis is that PRM/Alf milk contains intestinotrophic factors contributing to this remarkable phenotype. A comparison between the milk from PRM/Alf and C57BL/6J (as a control) strains could be helpful in the identification of such factors, including proteins. However, a complete description of the mouse milk major protein fraction is still missing. Hence we adapted a reliable technique to separate and identify the major mouse milk proteins. This approach was achieved through the protein study of milk from C57BL/6J and PWK/Pas strains representative of two Mus musculus subspecies, M. m. domesticus and M. m. musculus respectively. C57BL/6J milk samples were first skimmed and fractionated by reverse phase-HPLC (RP-HPLC). The protein content of each chromatographic peak was analysed by SDS-PAGE and identified by mass spectrometry. This methodological approach allowed characterization of nine major mouse milk proteins: alpha(s1), beta, gamma, epsilon and kappa-caseins, Whey Acidic Protein, lactoferrin, Serum Albumin, Fatty Acid Binding Protein, as well as an alpha(s1)-casein isoform. Then, RP-HPLC patterns of C57BL/6J milk proteins were compared with those obtained starting from the milk of PWK/Pas females. This comparison revealed a protein polymorphism for the alpha(s1)-casein.

http://www.ncbi.nlm.nih.gov/pubmed/19996491