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© Research
Publication : The Journal of allergy and clinical immunology

The 31 kd major allergen, Alt a I1563, of Alternaria alternata

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of allergy and clinical immunology - 01 Dec 1991

Paris S, Debeaupuis JP, Prévost MC, Casotto M, Latgé JP

Link to Pubmed [PMID] – 1744361

J. Allergy Clin. Immunol. 1991 Dec;88(6):902-8

A component of Alternaria extract, previously identified as the major allergen, Alt a I1563, was purified to homogeneity from Alternaria mycelium by means of acetone precipitation and ion-exchange chromatography. The homogeneity of Alt a I1563 was assessed by one single band after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by one single radiolabeled band after transfer to nitrocellulose, and by one single peak after size-exclusion chromatography by high-performance liquid chromatography. Alt a I1563 was isolated as a heat-stable acidic glycoprotein (carbohydrate content, 20%; pI, 4.0 to 4.5; 31 kd). The role of the carbohydrate moiety in the allergenicity is suggested. This major allergen is located in the cytoplasm of mycelium and spore.

http://www.ncbi.nlm.nih.gov/pubmed/1744361