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© Research
Publication : Proceedings of the National Academy of Sciences of the United States of America

Targeted replacement of the homeobox gene Hox-3.1 by the Escherichia coli lacZ in mouse chimeric embryos

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Proceedings of the National Academy of Sciences of the United States of America - 01 Jun 1990

Le Mouellic H, Lallemand Y, Brûlet P

Link to Pubmed [PMID] – 1972279

Proc. Natl. Acad. Sci. U.S.A. 1990 Jun;87(12):4712-6

Through gene targeting based upon homologous recombination in embryonic stem cells, a chosen gene can be inactivated and eventually a strain of mutant mice created. We have devised a procedure to specifically replace a targeted gene by another gene. A murine homeobox gene was disrupted at high frequency in embryonic stem cells by its replacement with Escherichia coli lacZ. Injection of such stem cells into blastocysts yielded chimeric embryos in which beta-galactosidase activity was driven by the Hox-3.1 promoter. This technique will allow the visual assessment at the cellular level of gene inactivation effects in transgenic mice.

http://www.ncbi.nlm.nih.gov/pubmed/1972279