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© Research
Publication : European journal of immunology

T cell dependence of the “natural” autoreactive B cell activation in the spleen of normal mice

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in European journal of immunology - 01 Oct 1988

Huetz F, Sciard-Larsson EL, Pereira P, Portnoi D, Coutinho A

Link to Pubmed [PMID] – 3263923

Eur. J. Immunol. 1988 Oct;18(10):1615-22

A high frequency of clonal precursor B cells producing lytic antibodies to syngeneic erythrocytes treated with bromelain (BMRC) is revealed in normal mouse spleen cells by lipopolysaccharide-driven limiting dilution analysis. All such specificities are recovered as activated blasts after density gradient fractionation, the “small lymphocyte” pool being depleted of anti-BMRC reactivities. In contrast, the spleens of athymic (nude) mice contain undetectably low frequencies of these specificities in either lymphocyte compartment. Transfer of relatively low numbers of normal syngeneic splenic T lymphocytes to adult nude mice restores the high frequency of anti-BMRC clonal precursor B cells, again in the activated, but not in the resting spleen cell fractions. Large total T cells are more than tenfold better than resting T cells in reconstitution potential, as are enriched CD4+ as compared to CD8+ cells which are practically devoid of activity in this respect. These results apply exclusively to B cells at a differentiative stage that allows for extensive clonal expansion, since there is a marked difference between the frequency of clonal precursors determined by limiting dilution analysis and the frequency of Ig-secreting plaque-forming cells of the same specificity, and induced by the same mitogen in short-term cultures. The implications of these findings for the physiology of autoreactivity and repertoire selection in the compartment of perinatal B cells are discussed.

http://www.ncbi.nlm.nih.gov/pubmed/3263923