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© Research
Publication : International immunology

Stromal cell independent growth of bipotent B cell–macrophage precursors from murine fetal liver

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in International immunology - 01 Mar 1994

Kee BL, Cumano A, Iscove NN, Paige CJ

Link to Pubmed [PMID] – 7514441

Int. Immunol. 1994 Mar;6(3):401-7

We have established stromal cell independent culture conditions under which single murine hematopoietic progenitor cells give rise to B lymphocytes and macrophages. IL-11, mast cell growth factor, and IL-7 are sufficient for the growth of B lineage cells from AA4.1+B220-Mac-1-Ly6A+ fetal liver cells isolated at day 12 of gestation. These conditions are also sufficient for commitment to myeloid differentiation, although CSF-1 is required for substantial growth of macrophages. The progenitors responding under these culture conditions: (i) require 8 days in culture before they become competent to respond to B cell mitogens in the presence of stromal cells and (ii) undergo a minimum of three cell divisions prior to Ig heavy chain allotype restriction. The findings suggest that the stromal cell dependency of immature hematopoietic cells may reflect a requirement for stromal cell derived growth factors. Further, this provides an in vitro system in which commitment and differentiation can be studied in single cells.

http://www.ncbi.nlm.nih.gov/pubmed/7514441