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© Research
Publication : Indian journal of microbiology

Statistical Optimization of Medium Components for Mass Production of Plant Growth-Promoting Microbial Inoculant Pseudomonas trivialis BIHB 745 (MTCC5336).

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Indian journal of microbiology - 01 Jun 2014

Vyas P, Rahi P, Chadha BS, Gulati A,

Link to Pubmed [PMID] – 25320429

Link to DOI – 10.1007/s12088-013-0425-9

Indian J Microbiol 2014 Jun; 54(2): 239-41

Optimizing nutritional requirements for mass production of microbial inoculants in shortened time has relevance for their economical field application. Therefore, the present study aimed at selecting suitable growth medium, optimizing its components, and up-scaling inoculum production for plant growth-promoting Pseudomonas trivialis BIHB 745. Of the different media tested, the culture exhibited maximal viable colony count in trypticase soya broth with 17.6 % increased biomass on optimizing levels of carbon source, nitrogen source, and NaCl using response surface methodology. A twofold higher biomass with 9 h shorter incubation period was obtained in optimized medium in a bioreactor in comparison to shake flasks.