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© Research
Publication : Indian journal of clinical biochemistry : IJCB

Standardization of a colorimetric method for the determination of fructose using o-cresol: Sulphuric acid reagent

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Indian journal of clinical biochemistry : IJCB - 01 Dec 1997

Kumar V, Pattabiraman TN

Link to Pubmed [PMID] – 23100873

Indian J Clin Biochem 1997 Dec;12(1):95-9

Fructose developed a pinkish orange chromogen on treatment with o-cresol: 70% sulphuric acid at 32°C for 15 minutes with a λ max of 500nm. Fructose was 185 times more chromogenic than glucose. Total carbohydrate and fructose values in protein-free filtrate of normal serum samples were in the range, 55.4-86.3 mg/dl and 1.55-3.29 mg/dl, respectively. In diabetes, the observed values were 197-354 mg/dl and 2.91-6.81 mg/dl, respectively.

http://www.ncbi.nlm.nih.gov/pubmed/23100873