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© MPI f. Dynamik und Selbstorganisation
Publication : Nature methods

Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

Team: Archived: Synapse and Circuit Dynamics
Member: David DiGregorio
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature methods - 30 Oct 2018

Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Müller JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL

Link to Pubmed [PMID] – 30377363

Nat. Methods 2018 Nov;15(11):936-939

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.