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© Research
Publication : Journal of clinical microbiology

Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of clinical microbiology - 09 Dec 2009

Njanpop Lafourcade BM, Sanou O, van der Linden M, Levina N, Karanfil M, Yaro S, Tamekloe TA, Mueller JE

Link to Pubmed [PMID] – 20007384

J. Clin. Microbiol. 2010 Feb;48(2):612-4

We reformulated a multiplex PCR algorithm for serotyping of pneumococcal meningitis directly on cerebrospinal fluid (CSF). Compared to established methods on isolates, CSF-based PCR had at least 80% sensitivity and 100% specificity. In regional meningitis surveillance, CSF-based PCR increased the serotype information yield from 40% of cases (isolate testing) to 90%.

http://www.ncbi.nlm.nih.gov/pubmed/20007384