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© Valérie Choumet
Mosquitoes were orally infected with the chikungunya virus. Midguts were dissected at day 5 post-infection, fixed and permeabilised. Virus is shown in red (anti-E2 protein, cyanine 3), the actin network in green (phalloidin 548) and nuclei in blue (DAPI).
Publication : Journal of clinical microbiology

Rapid differentiation of “Mycobacterium canettii” from other Mycobacterium tuberculosis complex organisms by PCR-restriction analysis of the hsp65 gene

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of clinical microbiology - 01 Oct 2001

Goh KS, Legrand E, Sola C, Rastogi N

Link to Pubmed [PMID] – 11574597

J. Clin. Microbiol. 2001 Oct;39(10):3705-8

A total of 102 isolates of the Mycobacterium tuberculosis complex, including available “M. canettii” isolates, were studied by PCR-restriction analysis of a 441-bp fragment of the hsp65 gene. PRA upon HhaI enzyme digestion (GCGC) allowed easy differentiation of “M. canettii” from other members of the M. tuberculosis complex (three bands of 260, 105, and 60 bp for “M. canetti,” compared to four bands of 185, 105, 75, and 60 bp for other members of the M. tuberculosis complex). Sequencing of the 441-bp hsp65 fragment of “M. canettii” isolates showed the disappearance of an HhaI site at position 235 due to a C-to-T transition that corresponded to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv. Considering that “M. canettii” may also exist as a stable rough morphotype, we suggest that the true number of “M. canettii” isolates may be underestimated in clinical microbiology laboratories.

http://www.ncbi.nlm.nih.gov/pubmed/11574597