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© Research
Publication : European journal of biochemistry / FEBS

Proteins bound to heterogeneous nuclear RNA of simian-virus-40-infected cells

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in European journal of biochemistry / FEBS - 01 Nov 1978

Pagoulatos GN, Yaniv M

Link to Pubmed [PMID] – 214301

Eur. J. Biochem. 1978 Nov;91(1):1-10

Heterogeneous nuclear RNA . protein (hnRNA . protein) complexes from simian-virus-40 (SV40)-infected cells late in infection contain 7–10% RNA sequences specific to SV40 DNA. The SV40 nuclear RNA . protein complexes sediment at 60–70 S. The reality and specificity of the RNA-protein association is shown in metrizamide gradients. Protein and RNA lebels of hnRNA . protein-particles in SV40-infected cells follow a parallel pattern with a peak at 1.28 g/cm2 whereas a mixture of ribosomal RNA and soluble cytoplasmic proteins is separated according to the different densities in metrizamide. Analysis of hnRNA . protein from infected cells by two-dimensional gel electrophoresis shows the presence of a number of new proteins. It is demonstrated that three of these proteins are cellular ones induced by the virus infection and hence constitute good candidates to be specific RNA . protein particles for virus nuclear RNA. The presence of actin in hnRNA . protein particles from normal and SV40-infected cells and the presence of the major capsid protein VP1 in hnRNA . protein particles from SV40-infected cells is discussed.

http://www.ncbi.nlm.nih.gov/pubmed/214301