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© Mart Krupovic, Junfeng Liu
Scanning electron micrograph of Saccharolobus islandicus cells (light blue) infected with the lemon-shaped virus STSV2 (yellow). Artistic rendering by Ala Krupovic.
Publication : Journal of virology

Protein-protein interactions leading to recruitment of the host DNA sliding clamp by the hyperthermophilic Sulfolobus islandicus rod-shaped virus 2

Team: Archaeal Virology
Member: Mart Krupovic
Member: David Prangishvili
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virology - 02 Apr 2014

Gardner AF, Bell SD, White MF, Prangishvili D, Krupovic M

Link to Pubmed [PMID] – 24696494

J. Virol. 2014 Jun;88(12):7105-8

Viruses infecting hyperthermophilic archaea typically do not encode DNA polymerases, raising questions regarding their genome replication. Here, using a yeast two-hybrid approach, we have assessed interactions between proteins of Sulfolobus islandicus rod-shaped virus 2 (SIRV2) and the host-encoded proliferating cell nuclear antigen (PCNA), a key DNA replication protein in archaea. Five SIRV2 proteins were found to interact with PCNA, providing insights into the recruitment of host replisome for viral DNA replication.