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© Valérie Choumet
Mosquitoes were orally infected with the chikungunya virus. Midguts were dissected at day 5 post-infection, fixed and permeabilised. Virus is shown in red (anti-E2 protein, cyanine 3), the actin network in green (phalloidin 548) and nuclei in blue (DAPI).
Publication : Chemical communications (Cambridge, England)

Programmed chemo-enzymatic synthesis of the oligosaccharide component of a carbohydrate-based antibacterial vaccine candidate

Team: Chemistry of Biomolecules
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Chemical communications (Cambridge, England) - 14 Feb 2015

Salamone S, Guerreiro C, Cambon E, André I, Remaud-Siméon M, Mulard LA

Link to Pubmed [PMID] – 25569152

Chem. Commun. (Camb.) 2015 Feb;51(13):2581-4

The powerful chemo-enzymatic synthesis of the pentadecasaccharide hapten involved in the first synthetic carbohydrate-based vaccine candidate against endemic shigellosis is reported. The high yielding site-selective α-D-glucosylation of a lightly protected disaccharide by an engineered transglucosylase-sucrose system gave a trisaccharide, which was chemically elongated by an efficient [5+5] process.