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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Annales de l'Institut Pasteur. Immunologie

Production of a monoclonal antibody against a major allergen of Dactylis glomerata pollen (Dg1)

Team: Biology of Host-parasite Interactions
Team: Archived: Group : Salah Mecheri
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Annales de l'Institut Pasteur. Immunologie - 01 Mar 1985

Mécheri S, Peltre G, Weyer A, David B

Link to Pubmed [PMID] – 2408551

Ann. Inst. Pasteur Immunol. 1985 Mar-Apr;136C(2):195-209

A mouse monoclonal antibody was produced against the most frequent allergen Ag Dg1ief, purified from the Dactylis glomerata grass pollen by isoelectric focusing. Using the immunoprint technique, this monoclonal antibody detected Ag Dg1ief in D. glomerata pollen and some cross-reacting molecules in four other grass pollens. This hybridoma was grown in ascitic fluid. An immunosorbent was made from it and, by affinity chromatography, was used to purify monoclonal Ag Dg1m from the pollen extract. Ag Dg1m (6,000 to 8,000 daltons) appeared to be 4 to 5 times smaller than Ag Dg1ief. It appeared as a weak allergen in passive cutaneous anaphylaxis in rats, but was more potent in triggering histamine release on human basophils from grass-pollen sensitive patients. These results suggest that Ag Dg1m could be a part of the Ag Dg1ief used to induce production of this monoclonal antibody.