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© Research
Publication : Nature methods

Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature methods - 18 May 2015

Kaplan M, Cukkemane A, van Zundert GC, Narasimhan S, Daniëls M, Mance D, Waksman G, Bonvin AM, Fronzes R, Folkers GE, Baldus M

Link to Pubmed [PMID] – 25984698

Nat. Methods 2015 Jul;12(7):649-52

Studying biomolecules at atomic resolution in their native environment is the ultimate aim of structural biology. We investigated the bacterial type IV secretion system core complex (T4SScc) by cellular dynamic nuclear polarization-based solid-state nuclear magnetic resonance spectroscopy to validate a structural model previously generated by combining in vitro and in silico data. Our results indicate that T4SScc is well folded in the cellular setting, revealing protein regions that had been elusive when studied in vitro.

http://www.ncbi.nlm.nih.gov/pubmed/25984698