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© Research
Publication : Annales de l'Institut Pasteur. Microbiologie

Preparation of tuberculin antigen L

Member: Pascale Pescher
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Annales de l'Institut Pasteur. Microbiologie - 01 Sep 1985

Romain F, Versmisse E, Pescher P, Augier J

Link to Pubmed [PMID] – 4083836

Ann. Inst. Pasteur Microbiol. 1985 Sep-Oct;136B(2):235-48

The preparation of antigen L using culture filtrates of live BCG is described. The procedure consisted essentially of precipitating this filtrate with 10% trichloroacetic acid in the presence of N-butyl alcohol 4% final concentration. The precipitate was chromatographed on carboxy-methyl-trisacryl-M and then on DEAE-trisacryl-M; in both cases, maximal biological activity (delayed-type hypersensitivity) was found in the void volume. The product thus obtained was chromatographed on a molecular sieve so as to eliminate poorly active fractions. The final product was 30-fold more active on guinea-pigs sensitized with live BCG than on those sensitized with inactivated BCG.