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© Research
Publication : Cell

Preferential association of newly synthesized histones with replicating SV40 DNA

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Cell - 01 Dec 1977

Crémisi C, Chestier A, Yaniv M

Link to Pubmed [PMID] – 202394

Cell 1977 Dec;12(4):947-51

The assembly of newly synthesized histones into nucleosomes during replication of SV40 minichromosomes in vivo was studied. Infected cells were labeled with 35S-methionine for a time shorter than that required to complete a round of viral DNA replication. Mature and replicating SV40 minichromosomes were extracted and separated by zonal sedimentation, and their histone content was analyzed by polyacrylamide gel electrophoresis (SDS and acidic urea). We show that the pulse-labeled histones associate preferentially with the replicating DNA.

http://www.ncbi.nlm.nih.gov/pubmed/202394